human eoc cell line sk-ov-3  (Procell Inc)

Journal: bioRxiv

Article Title: Developing a Multimodal miR-15a Mimic to Overcome PARP Inhibitor Resistance in Epithelial Ovarian Cancer

doi: 10.64898/2026.04.20.719456

Figure Lengend Snippet: (A) Schematic representation of hsa-miR-15a and MTX-5-FU-Gem-miR-15a, showing incorporation of 5-fluorouracil and gemcitabine into the miR-15a backbone and conjugation of methotrexate (MTX) to the passenger strand. (B-E) Dose-response curves showing cell viability following treatment with MTX-5-FU-Gem-miR-15a, unmodified miR-15a, and olaparib in SK-OV-3 (B), OVCAR-3 (C), A2780 (D), and UWB1.289 (E) cells. Data represent mean ± SD from n = 4 biological replicates.

Article Snippet: Human epithelial ovarian cancer (EOC) cell lines SK-OV-3, OVCAR-3, A2780, and UWB1.289 were obtained from the American Type Culture Collection (ATCC) and MilliporeSigma.

Techniques: Conjugation Assay

Journal: bioRxiv

Article Title: Developing a Multimodal miR-15a Mimic to Overcome PARP Inhibitor Resistance in Epithelial Ovarian Cancer

doi: 10.64898/2026.04.20.719456

Figure Lengend Snippet: (A) Representative histograms of DNA content (PI staining) in only negative control and MTX-5-FU-Gem-miR-15a treatment showing cell cycle distribution in SK-OV-3, OVCAR-3, A2780, and UWB1.289 cells following treatment. (B) Quantification of cell cycle distribution after treatment shown as fold change in G2/S ratio relative to negative control across cell lines. (C) Representative Annexin V/PI flow cytometry plots showing apoptosis in indicated cell lines. (D) Quantification of apoptotic cells (early + late apoptosis) shown as fold change relative to control. (E) Western blot analysis showing expression of cleaved PARP, BCL-2, BAX, and BAK following treatment; β-actin serves as a loading control. Data represent mean ± SD from n = 3 biological replicates. Statistical significance between two groups was determined using two-tailed Student’s t test. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Human epithelial ovarian cancer (EOC) cell lines SK-OV-3, OVCAR-3, A2780, and UWB1.289 were obtained from the American Type Culture Collection (ATCC) and MilliporeSigma.

Techniques: Staining, Negative Control, Flow Cytometry, Control, Western Blot, Expressing, Two Tailed Test

Journal: bioRxiv

Article Title: Developing a Multimodal miR-15a Mimic to Overcome PARP Inhibitor Resistance in Epithelial Ovarian Cancer

doi: 10.64898/2026.04.20.719456

Figure Lengend Snippet: (A, C, E) Western blot analysis of canonical miR-15a target proteins in ovarian cancer cells treated with negative control, miR-15a, MTX-5-FU-Gem-miR-15a, or 5-FU + gemcitabine. Panel A, SK-OV-3; panel C, OVCAR-3; panel E, UWB1.289. (B, D, F) Densitometric quantification of the corresponding western blots normalized to β-actin. Data represent mean ± SD from n = 3 biological replicates. Statistical significance between two groups was determined using two-tailed Student’s t test. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Human epithelial ovarian cancer (EOC) cell lines SK-OV-3, OVCAR-3, A2780, and UWB1.289 were obtained from the American Type Culture Collection (ATCC) and MilliporeSigma.

Techniques: Western Blot, Negative Control, Two Tailed Test

Journal: bioRxiv

Article Title: Developing a Multimodal miR-15a Mimic to Overcome PARP Inhibitor Resistance in Epithelial Ovarian Cancer

doi: 10.64898/2026.04.20.719456

Figure Lengend Snippet: (A-C) Dose-response curves for MTX-5-FU-Gem-miR-15a and olaparib in olaparib-resistant OVCAR-3/OlaR (A), SK-OV-3/OlaR (B), and UWB1.289/OlaR (C) cells. (D) Representative cell cycle histograms (PI staining) and quantification of fold change in G2/S ratio in resistant cell lines. (E) Representative Annexin V/PI plots and quantification of total apoptotic cells (early + late apoptosis). (F-G) Dose-response analysis of cell viability in OVCAR-3 (F) and SK-OV-3 (G) spheroid cultures treated with increasing concentrations of MTX-5-FU-Gem-miR-15a or olaparib. (H) Western blot analysis of WEE1, CHK1, CCND1, and BCL-2 in OVCAR-3 spheroid cultures following treatment; β-actin serves as the loading control. Data represent mean ± SD from n = 3 biological replicates. Statistical significance between two groups was determined using two-tailed Student’s t test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Human epithelial ovarian cancer (EOC) cell lines SK-OV-3, OVCAR-3, A2780, and UWB1.289 were obtained from the American Type Culture Collection (ATCC) and MilliporeSigma.

Techniques: Staining, Western Blot, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Developing a Multimodal miR-15a Mimic to Overcome PARP Inhibitor Resistance in Epithelial Ovarian Cancer

doi: 10.64898/2026.04.20.719456

Figure Lengend Snippet: (A) Schematic of the in vivo experimental design and treatment schedule. (B) Representative bioluminescence images of parental SK-OV-3 xenografts at endpoint. (C) Tumor growth curves over time for vehicle- and MTX-5-FU-Gem-miR-15a-treated parental xenografts. (D) Kaplan-Meier survival analysis of parental xenografts. (E) Serum AST and ALT measurements evaluating treatment-associated toxicity. (F) Representative bioluminescence images of resistant SK-OV-3/OlaR xenografts treated with vehicle, intravenous MTX-5-FU-Gem-miR-15a, or intraperitoneal MTX-5-FU-Gem-miR-15a. (G) Tumor growth curves over time for resistant xenografts. (H) Proposed mechanistic model of MTX-5-FU-Gem-miR-15a activity. Data represent mean ± SD. Statistical significance for tumor growth and body weight analyses was determined using two-way ANOVA with appropriate post hoc testing. Survival differences were analyzed using the log-rank (Mantel-Cox) test. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Human epithelial ovarian cancer (EOC) cell lines SK-OV-3, OVCAR-3, A2780, and UWB1.289 were obtained from the American Type Culture Collection (ATCC) and MilliporeSigma.

Techniques: In Vivo, Activity Assay

Journal: International Journal of Biological Sciences

Article Title: DHCR24 Drives Ovarian Cancer Chemoresistance Through Lipid Raft-mediated P-gp Stabilization and STAT3 Activation

doi: 10.7150/ijbs.128173

Figure Lengend Snippet: DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and SKOV3/DDP cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.

Article Snippet: Human EOC cell lines SKOV3 and A2780 were obtained from ATCC (American Type Culture Collection), while their drug-resistant counterparts, SKOV3/DDP and A2780/DDP, were acquired from Wuhan Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot, Knockdown, Real-time Polymerase Chain Reaction, Disruption, Immunofluorescence

Journal: International journal of molecular sciences

Article Title: YKL40/Integrin β4 Axis Induced by the Interaction between Cancer Cells and Tumor-Associated Macrophages Is Involved in the Progression of High-Grade Serous Ovarian Carcinoma.

doi: 10.3390/ijms251910598

Figure Lengend Snippet: Figure 2. Co-culture of macrophages and epithelial ovarian cancer (EOC) cells promotes polarization of macrophages into TAMs and enhances malignant phenotypes of EOC cells. (A): Indirect co-culture of EOC cells (KURAMOCHI, SKOV3 and OVCAR3) in the lower chamber and macrophages in the transwell assay with 0.4 µm pores was performed for 48 h. For comparison, EOC cells and macrophages were also cultured alone for 48 h as monocultured controls. (B): Expression of TAM markers CD163 and CD204 was compared using real-time quantitative PCR (qPCR) between mono- cultured and co-cultured macrophages. (C): Expression of extracellular signal-regulated kinase (Erk) and phosphorylated Erk (p-Erk; Thr202/Tyr204) in monocultured and co-cultured EOC cells was evaluated using Western blotting. β-actin was used as a control. (D): MTS assays were performed to compare the proliferation of monocultured and co-cultured EOC cells. (E): Transwell migration assays were performed to compare monocultured and co-cultured EOC cells. After 48 h of incubation, migrating cells into the lower surface were counted in five random fields per chamber. Representative images are shown in Figure S2A. (F): Transwell invasion assays were performed to compare mono- cultured and co-cultured EOC cells. After 48 h of incubation, invading cells into the lower surface were counted in five random fields per chamber. Representative images are shown in Figure S2B. Mϕ, macrophages. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Three human EOC cell lines (KURAMOCHI, SKOV3, and OVCAR3) were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), American Type Culture Collection (Manassas, VA, USA), and Biological Resource Center (Tsukuba, Japan), respectively, and maintained in RPMI 1640 medium (Wako, Osaka, Japan) with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA) and 1% antibiotic/antimycotic stock solution (Wako) at 37 ◦C in a 5% CO2 atmosphere.

Techniques: Co-Culture Assay, Transwell Assay, Comparison, Cell Culture, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control, Migration, Incubation

Journal: International journal of molecular sciences

Article Title: YKL40/Integrin β4 Axis Induced by the Interaction between Cancer Cells and Tumor-Associated Macrophages Is Involved in the Progression of High-Grade Serous Ovarian Carcinoma.

doi: 10.3390/ijms251910598

Figure Lengend Snippet: Figure 3. Increased YKL40 resulting from indirect co-culture of macrophages and EOC cells promotes malignant phenotypes of EOC cells. (A): Macrophages and EOC cells were each monocultured for 48 h, and macrophages and EOC cells were co-cultured for 48 h. Cell supernatants were collected. (B): Cytokine arrays were performed on each collected cell supernatant. Colored boxes indicate spots of enhanced signal in the supernatants of the co-culture compared with the KURAMOCHI and macrophage monocultures. (C): ELISA was performed to investigate the secretion of YKL40 in three types of cell supernatants for each EOC cell line. (D–F): Each EOC cell line was treated with recombinant human YKL40 (rhYKL40) at concentrations of 0, 250 and 500 ng/mL, which was performed for (D) MTS assays, (E) transwell migration assays, and (F) transwell invasion assays after 48 h. (E) Migrating cells and (F) invading cells were counted in five random fields per chamber. Representative images of transwell migration and invasion assays are shown in Figure S2C,D, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001. N.S., not significant.

Article Snippet: Three human EOC cell lines (KURAMOCHI, SKOV3, and OVCAR3) were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), American Type Culture Collection (Manassas, VA, USA), and Biological Resource Center (Tsukuba, Japan), respectively, and maintained in RPMI 1640 medium (Wako, Osaka, Japan) with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA) and 1% antibiotic/antimycotic stock solution (Wako) at 37 ◦C in a 5% CO2 atmosphere.

Techniques: Co-Culture Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Recombinant, Migration